Diyaarinta iyo Daraasada IgY Antibodies ilaa Motif Common of Metallase ee Agkistrodon acutus Venom

IgY antibody ka soo horjeeda motif guud ee metalloproteinase ka Agkistrodon acutus venom ayaa la diyaariyey iyo doorka ay ku leedahay dhaqdhaqaaqa sunta abeesooyinka ayaa la darsay [Habab] Anagoo falanqeynaya taxanaha caadiga ah ee metalloproteinases kala duwan ee Agkistrodon acutus venom, waxaan naqshadeynay oo aan soo saarnay peptides antigenic PT21 iyo PT2. , kuwaas oo si dhow ula xiriira hawlahooda enzyme;Digaagga waxaa lagu tallaalay dhismooyinka isku dhafan ee KLH-PT1 iyo KLH-PT2 si loo helo unugyada difaaca IgY.Titer of IgY iyo sifooyinka falcelinta iskutallaabta leh sunta Agkistrodon acutus iyo Agkistrodon brevius waxaa si horudhac ah u bartay ELISA iyo Western blot;Ugu dambeyntii, waxqabadka dhexdhexaadinta ee IgY waxaa lagu qiimeeyay tijaabada dhiig-baxa subcutaneous ee jiirarka [Natiijooyinka] ELISA iyo Western blot waxay muujisay in anti-KLH-PT1 iyo anti Agkistrodon acutus venom IgY ay ka gudbi karaan falcelinta Agkistrodon acutus venom iyo Agkistrodon brevius venom, iyo kan dambe. ayaa si weyn uga xoog badnaa kii hore;Kahortagga KLH-PT2 IgY ma lahayn falcelin isdhaafsi oo muhiim ah Agkistrodon acutus iyo Agkistrodon brevius venoms in vitro, laakiin waxay muujisay sunta ka takhalusida sunta adag ee tijaabada dhiigbaxa ka hortagga ee vivo, waxayna aad uga sareysay labadii hore [Gabagabo] IgY antibody kaas oo awood u leh iskutallaabtu waxay ka falcelisay dhiig kala duwan oo wareegaya sunta abeesooyinka waxaa la diyaariyey iyadoo la naqshadeeyay antigen peptide-ka ay wadaagaan metalloproteinases, kaas oo dhidibbada u taagay diyaarinta gaar ahaan, hufan, suntan hoose iyo spectrum ballaaran oo ka hortagga dhiigbaxa sunta maska.