[Ujeedo] Si aad u diyaariso IgY antibody ka soo horjeeda ujeedka guud ee metalloproteinases ee ka yimaada Agkistrodon acutus venom, iyo in la barto saamaynta ay ku leedahay waxqabadka sunta maska [hababka] Anagoo falanqeynaya taxanaha caadiga ah ee noocyada kala duwan ee metalloproteinase ee Agkistrodon acutus venom, waxaan naqshadeynay oo la farsameeyey peptides antigen-ka aadka loo ilaaliyo ee PT1 iyo PT2, kuwaas oo si dhow ula xidhiidha hawlaha enzyme;Digaagga waxaa lagu tallaalay isku-dhisyada isku-dhafan ee KLH-PT1 iyo KLH-PT2 si loo helo unugyada difaaca IgY.Titer-ka IgY iyo sifooyinka falcelinta falcelinta ee Agkistrodon acutus iyo Agkistrodon acutus venom waxaa bartay ELISA iyo Western blot;Ugu dambeyntii, dhaqdhaqaaqa dhexdhexaadinta ee IgY waxaa lagu qiimeeyay tijaabada dhiig-baxa subcutaneous ee jiirarka [Natiijooyinka] Natiijooyinka ELISA iyo Western blot waxay muujiyeen in anti-KLH-PT1 iyo anti-Agkistrodon acutus IgY ay ka gudbi karaan falcelinta Agkistrodon acutus iyo Agkistrodon acutus venom , kan dambena aad buu uga xoog badnaa kuwii hore;Anti-KLH-PT2 IgY ma laha falcelin cad oo iskutallaab ah oo leh Agkistrodon acutus venom iyo Agkistrodon acutus venom in vitro, laakiin waxay leedahay sunta ka takhalusida adag ee tijaabada dhiig-baxa ee vivo, waxayna aad uga sareysaa labadii hore [Gabagabo] IgY antibody oo ka falcelin kara dhiig kala duwan oo wareegaya sunta abeesooyinka ayaa la diyaariyey iyadoo la naqshadeeyay taxanaha antigen peptide ee metalloproteinase, kaas oo dhidibada u aasay diyaarinta sun gaar ah, hufan, hoosaysa iyo spectrum ballaaran anti-hemorrhagic anti-hemorrhagic venom antibody.
Waqtiga boostada: Jan-12-2023